Cell and Developmental Biology
Teaching: Biol 3043 - Cell Biology, Biol 3162 - Developmental Biology, Biol 4182 - Experimental Embryology, and occasionally Biol 2023 - Introductory Biochemistry.
Tissues are comprised of a population of cells embedded in a complex matrix of large glycoproteins that contribute significantly to both the mechanical and biological properties of the tissue. During embryonic development, and physiological as well as pathological tissue remodeling in adults, cells secrete proteases that degrade this extracellular matrix, allowing them to re-synthesize it in a new configuration. The regulation of this degradation and re-synthesis is of fundamental importance not only during embryonic development, but also the homeostasis of normal adult tissues, and its mis-regulation underlies a plethora of pathologies. Research in my lab utilizes the zebrafish embryo as a model system in which we can manipulate the molecules responsible for these activities and observe their activities using novel techniques such as the EMMA assay, in vivo activity profiling, in vivo zymography, and xenotransplantation, in addition to well-established molecular and cell-biological approaches.
Jeffrey, E.J.J. and Crawford, B.D. (2018) "The Epitope Mediated MMP Activation assay: detection and quantification of the activation of Mmp2 in vivo in the zebrafish embryo” In press. Histochemistry and Cell Biology. doi:10.1007/s00418-018-1634-4
Wyatt, R.A., Trieu, N.P.V. and Crawford B.D. (2017) “Zebrafish xenograft: an evolutionary experiment in tumour biology.” Genes 8:220 doi:10.3390/genes8090220
Ellis, T.R. and Crawford, B.D. (2016) “Experimental Dissection of Metalloproteinase Inhibition-Mediated and Toxic Effects of Phenanthroline on Zebrafish Development.” International Journal of Molecular Science 17(9):1503-1520 doi:10.3390/ijms17091503
Jenkins, M.H., Alrowaished, S.S., Goody, M.F., Crawford, B.D. and Henry, C.A. (2016) "Laminin and Matrix metalloproteinase 11 regulate Fibronectin levels in the zebrafish myotendinous junction." Skeletal Muscle 6(1):18 doi:10.1186/s13395-016-0089-3
Small, C.D. and Crawford, B.D. (2016). "Matrix metalloproteinases in neural development and regeneration: a phylogenetically and experimentally diverse perspective." Neural Regeneration Research 11(3):357-362. doi:10.4103/1673-5374.179030
Crawford, B.D., Po, M.D., Forsberg, D., Saranyan, P.V., Schulz, R. and Pilgrim, D.B. (2014). "Mmp25b facilitates elongation of sensory neurons during zebrafish development." Genesis: the Journal of Genetics and Development. 52(10):833-848. doi:10.1002/dvg.22803
Keow, J. Y., & Crawford, B. D. (2012). "Investigating Matrix Metalloproteinase Regulation in its Biological Context; Detecting MMP Activity In Vivo." In B. A. Sorg & T. E. Brown (Eds.), Matrix Metalloproteinases: (pp. 151–169).
Keow, J.Y, Cisar, J.S., Cravatt, B.F., and Crawford, B.D. (2012). "In vivo activity-based labeling of matrix metalloproteinases in vertebrate embryos." PLoS One. 7:e43434. doi:10.1371/journal.pone.0043434
Goody, M.F., Kelly, M.W., Reynolds, C.J., Khalil, A.J., Crawford, B.D., and Henry, C.A. (2012). "NAD+ Biosynthesis ameliorates muscular dystrophy." PLoS Biology. 10:e1001409 doi:10.1371/journal.pbio.1001409
Ali, M.A.M, Chow, A.K., Kandasamy, A.D., West, L.J., Crawford, B.D., and Schulz, R. (2012). "Mechanisms of cytosolic targeting of matrix metalloproteinase-2." Journal of Cellular Physiology. 227:3397–404. doi:10.1002/jcp.24040
Keow, J.Y, Herrmann, K.M., and Crawford, B.D. (2011). "Differential in vivo zymography: a method for observing matrix metalloproteinase activity in the zebrafish embryo." Matrix Biology 30:169-77. doi:10.1016/j.matbio.2011.01.003
Wyatt, R.A., Keow, J.Y, Haché, C., Harris, N.D. and Crawford, B.D. (2009) "The zebrafish embryo: a powerful model system for investigating matrix remodeling." Zebrafish 6:347-54. doi:10.1089/zeb.2009.0609
Nhu Trieu (M.Sc.): Regulation of ECM remodeling at the host-tumour interface using xenografts
Rachael Wyatt (Ph.D.): Analysis of the activation of Mmp2 during zebrafish development in vivo
Christopher Small (Ph.D.): Analysis of the effects of ploidy on erythropoiesis using transgenic zebrafish as a model system.
Emma Jeffery (M.Sc.): Post-translational activation of matrix metalloproteinases revealed by epitope tagged fusion proteins in vivo.
Emma Matchett (M.Sc.): Regulation of Stromelysin-3 by Timp4 at maturing myotome boundaries.
Amina Fallata (M.Sc.): Intracellular functions of Mmp2 in skeletal muscle and its regulation by phosphorylation.
Jonathan Keow (M.Sc.): Activity based detection of metalloproteinase activity during zebrafish development.
Natalie Harris (M.Sc.): The effects of gravity on the expression of ECM remodeling enzymes during zebrafish development.