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CONFOCAL LASER SCANNING MICROSCOPE
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Zebrafish image: Dr. Bryan Crawford, Biology, UNB
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INSTRUMENTATION
Confocal Laser Scanning Microscope with:
- Leica DM IRE2 inverted stand IRE2 light microscope.
- Wide selection of objective lenses including
multi-immersion and long working-distance.
- 4 lasers; Argon/Krypton; HeNe (green); HeNe (orange);
HeNe (red).
- 8 excitation wavelengths: 457, 476, 488, 496,
514, 543, 594, 633.
- 5 detectors - 4 fluorescent channels plus one
transmitted light detector.
- AOBS (programmable beam splitter).
- Physiology and materials software.
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Rotating projections of composite confocal stacks
through transgenic zebrafish embryos.
Green
fluorescent protein (GFP) in developing endothelial
cells.
Top embryo, 24 hpf; middle embryo 48 hpf; bottom
embryo, 96 hpf.
Class of Biology 3181, UNB Biology, Winter 2011.
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