|C.L. Patten, Ph. D. (Waterloo), Post-Doctorate (McMaster)
Biochemical and genetic characterization of beneficial plant-bacterial interactions
Many soil bacteria promote the growth of healthy plants and the research in my lab aims to understand how they do this at the biochemical and genetic level. We have determined that secretion of the plant hormone indoleacetic acid by some common soil bacteria is required for stimulation of host root development and are investigating the regulation of indoleacetic acid biosynthesis in response to environmental signals. We are also using genomics approaches to identify other bacterial genes that function in stimulation of plant growth and that enhance competitive colonization of roots. Plant growth-promoting bacteria introduced into agricultural soils often do not compete effectively with indigenous microbes for plant colonization. In the long term, knowledge of the molecular basis for beneficial plant-bacterial interactions can be applied to increase yields of economically important plants.
Henderson, S.L., Dandie, C.E., Patten, C.L., Zebarth, B.J., Burton, D.L., Trevors, J.T., and Goyer, C. 2010. Changes in denitrifier abundance, denitrification gene mRNA levels, nitrous oxide emissions and denitrification in anoxic soil microcosms amended with glucose and plant residues. Applied and Environmental Microbiology, 76:2155-2164.
English, M.M., Coulson, T.J.D., Horsman, S.R., and Patten, C.L. 2010. Overexpression of hns in the plant growth-promoting bacterium Enterobacter cloacae UW5 increases root colonization. Journal of Applied Microbiology, 108:2180-2190.
Glick, B.R., Pasternak, J.J. and Patten, C.L. 2010. Molecular Biotechnology: Principles and Applications of Recombinant DNA, 4th edition. American Society for Microbiology Press, Washington, D.C.
Wertz, S., Dandie, C.E., Goyer, C., Trevors, J.J., and Patten, C.L. 2009. Diversity of nirK denitrifying genes and transcripts in an agricultural soil. Applied and Environmental Microbiology, 75:7365-7377.
Ryu, R.J., and Patten, C.L. 2008. Aromatic amino acid-dependent expression of indole-3-pyruvate decarboxylase is regulated by TyrR in Enterobacter cloacae UW5. Journal of Bacteriology, 190:7200-7208.
Patten, C.L. and Glick, B.R. 2008. Plant growth-promoting bacteria: significance in horticulture. In: Microbial Biotechnology in Horticulture, Vol. 2, (eds) Ray, R.C. and Ward, O.P., Science Publishers, Enfield, N.H., USA, pgs. 145-176.
Cowie, A., Cheng, J., Sibley, C.D., Fong, Y., Zaheer, R., Patten, C.L., Morton, R.M., Golding, G.B. and Finan, T.M. 2006. An integrated approach to functional genomics: construction of a novel reporter gene fusion library of Sinorhizobium meliloti. Applied and Environmental Microbiology, 72:7156-7167.
Patten, C.L., Kirchhof, M.G., Schertzberg, M.R., Morton, R.A. and Schellhorn, H.E. 2004. Microarray analysis of RpoS-mediated gene expression in Escherichia coli K-12. Molecular Genetics and Genomics, 272: 580-591.
Vijayakumar, S.R.V., Kirchhof, M.G., Patten, C.L. and Schellhorn, H.E. 2004. RpoS-regulated genes of Escherichia coli identified using random lacZ fusion mutagenesis. Journal of Bacteriology, 186: 8499-8507.
Chen, G., Patten, C.L. and Schellhorn, H.E. 2004. Positive selection for loss of RpoS function in Escherichia coli. Mutation Research, 554: 193-203.
Chen, G., Patten, C.L. and Schellhorn, H.E. 2003. Controlled expression of an rpoS antisense RNA can inhibit RpoS function in Escherichia coli. Antimicrobial Agents and Chemotherapy, 47: 3485-3493.
Patten, C.L. and Glick, B.R. 2002. The role of Pseudomonas putida indoleacetic acid in the development of the host plant root system. Applied and Environmental Microbiology, 68: 3795-3801.
Patten, C.L. and Glick, B.R. 2002. Regulation of Pseudomonas putida indoleacetic acid production by tryptophan and the stationary phase sigma factor RpoS. Canadian Journal of Microbiology, 48: 635-642.
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Last Update: 22 July 2010
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